By T.S. Work and E. Work (Eds.)
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Extra resources for Density Gradient Centrifugation
Aerosol formation A serious problem sometimes encountered with zonal rotors is the formation of aerosols (or atomised leaks). In the case of radioactive samples or infectious viruses, stringent control must be observed. If the rotating seal leaks, most of the material is thrown out onto the guard tray and the centrifuge bowl, forming a distinct ring which can later be washed or decontaminated. However, an unknown quantity of the leaked material will undoubtedly atomize and spread quickly in the atmosphere.
Safety of centrifuges Centrifuges can be dangerous if wrongly used or improperly maintained. The safety factor is strongly linked to care of machine and rotors, and it is therefore logical to discuss the two together. I t cannot be emphasised too strongly that carelessness will increase the chance of injury and lead to higher expenditure on repair and replacement. Fortunately centrifuge manufacturers do ensure that the possibility of injury is minimised. To avoid accidents all users of centrifuges should be persuaded to read the manuals and other publications referring to care and safety (Price 1972; Burns 1972) as part of a training scheme.
We have replaced these bolts with high-tensile steel bolts, which have proved to be much more satisfactory. They are not stainless, but are regularly greased and show no sign of rusting after four year's use. B rotors. These are of simple construction and are so easy to clean and maintain that they can be washed, dried and put away in 5 min. Silicone grease should be applied only sparingly to the centre core O-rings, as excess grease can block the four holes leading down the vanes. If these holes do get blocked or contaminated with fungal growth, they can be cleaned effectively with a pipe cleaner wetted with a detergent and pushed down the whole length of the vane.
Density Gradient Centrifugation by T.S. Work and E. Work (Eds.)