By G. Alan Robison
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1970). These investigators used high pressure disintegration iri the presence of phosphatidylethanolamine to obtain a "solubilized" preparation of adenyl cyclase from an adrenal tumor. This preparation could be fractionated with the retention of sensitivity to stimulation by ACTH. The relation between ACTH-binding and the stimulation of adenyl cyclase activity was very striking in these experiments, emphasizing further the close relation that exists between hormone receptors and adenyl cyclase.
It is equally clear that if a response occurs in the absence of a change in the level of cyclic AMP, then the response cannot be mediated by cyclic AMP. As with broken cell preparations, possible complications arising from different cell types need always to be kept in mind. Adipose tissue, for example, contains cells which respond to prostaglandin E x with an increase in the level of cyclic AMP, as well as true adipocytes which respond with a fall in the level of cyclic AMP (Chapter 8). As a result, the relation of the antilipolytic effect of prostaglandin E i to its effect on the level of cyclic AMP in adipose tissue could not be understood until studies were carried out with isolated fat cells.
In certain other cases opposite changes in the level of cyclic AMP may occur in response to the same hormone acting under different conditions. Epinephrine, for example, is capable of interacting in some tissues with two different types of receptors. Interaction with one type leads to an increase in the level of cyclic AMP while interaction with the other leads to a decrease, and the net effect of epinephrine in these tissues will depend on whichever type of receptor predominates. This is discussed in more detail in Chapter 6.
Cyclic AMP by G. Alan Robison