By John M. Lowenstein
The significantly acclaimed laboratory typical, Methods in Enzymology, is among the so much hugely revered courses within the box of biochemistry. considering 1955, each one quantity has been eagerly awaited, often consulted, and praised by means of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless appropriate this present day - really a necessary ebook for researchers in all fields of existence sciences
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193, 265 (1951). P. D. J. Weitzman and P. Dunmore, unpublished. ~H. L. Kornberg, P. J. R. Phizackerley, and J. R. Sadler, Biochem. 77, 438 (1960). 24 aEACTIONS ON THE CYCLE  protein), and the mixture is stirred continuously. After 30 minutes the precipitate is removed by centrifugation for 20 minutes at 25,000 g. Step 3. Fractionation with Ammonium Sul]ate. 1 g of finely ground solid (NH4)2SO~ (55% saturation). After the preparation has stood for 30 minutes with continuous stirring, the precipitate is removed by centrifugation for 20 minutes at 25,000 g.
E. Atkinson, J. Biol. Chem. 238, 2875 (1963). ~ R. F. Chert and G. W. E. Plaut, unpublished observation, 1963. ~H. Goebell and M. Klingenberg, Biochem. Z. 340, 441 (1964). R. F. Chen and G. W. E. Plaut, Biochemistry 2, 752 (1963). ~7G. E. Lienhard and I. A. Rose, Biochemistry 3, 185 (1964). ~*S. Englard and S. P. Colowick, J. Biol. Chem. 226, 1047 (1957). S. Englard, J. Biol. Chem. 235, 1510 (1960). ~°S. Englard and I. Listowsky, Biochem. Biophys. Res. Commun. 12, 356 (1963). G. W. E. -C. Sung.
0 with concentrated ammonium hydroxide. Percent saturation is calculated on the basis of solubility at 25 ° . , by the method of Johnson'). M. J. Johnson, in "Manometric Techniques and Related Methods for the Study of Tissue Metabolism" (W. W. Umbreit, R. H. Burris, and J. F. , p. 161. Burgess, Minneapolis, Minnesota, 1949. 36 REACTIONS ON THE CYCLE  by eentrifugation and suspended in 60 ml of 3 0 3 saturated ammonium sulfate solution. Step ~. Dialysis. To a 20-ml aliquot of the enzyme suspension from step 3 is added 8 ml of saturated ammonium sulfate solution.
Citric Acid Cycle by John M. Lowenstein