By T. Sreevalsan (auth.), Ronald L. Cihlar, Richard A. Calderone (eds.)
Over the process the prior decade, there were striking advances within the examine of human pathogenic fungi. those advancements have taken position all through a variety of disciplines, and feature come because the results of newly to be had genome sequences of pathogens resembling candida albicans and different version fungi. In Candida Albicans: equipment and Protocols, specialist researchers discover those interesting new insights, targeting the examine of medically very important fungi and Candida spp particularly. Chapters research serious features of molecular equipment, supplying details on reporter gene assays, transformation, gene expression in vivo, and strategies for large-scale gene disruption. while, the paintings comprises in-depth descriptions of illness versions of candidiaisis, proof approximately pressure id, and directions at the guidance of samples for proteomic investigations and tandem affinity purification. Composed within the hugely profitable Methods in Molecular Biology™ sequence structure, every one bankruptcy features a short advent, step by step equipment, an inventory of useful fabrics, and a Notes part which stocks tips about troubleshooting and keeping off identified pitfalls.
Authoritative and leading edge, Candida Albicans: tools and Protocols is a useful resource of equipment for investigators within the exhilarating fields of clinical and molecular mycology.
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Additional resources for Candida albicans: Methods and Protocols
And Whiteway, M. 7 mouse macrophage cells: Effects of Candida genotype, infection ratios, and gamma interferon treatment. Infect. Immun. 70, 6319–6329. 5. Collins, S. , Ruscetti, F. , Gallagher, R. , and Gallo, R. C. (1979) Normal functional characteristics of cultured human promyelocytic leukemia cells (HL60) after induction of differentiation by dimethylsulfoxide. J. Exp. Med. 149, 969–974. 6. , and Ralph, R. (1985) Human leukemic models of myelomonocytic development: A review of the HL60 and U937 cell lines.
Immunization Protocols for Protection Against Candidiasis 31 Growth of C. albicans strains for infection. 5% glucose (dextrose)), overnight, then standardized to a specific inoculum using a hemocytometer (see Appendix chapter). v. with either 104 or 105 viable C. p. inoculation with live C. 2 mL saline or 5% mucin); (c) immunosuppressed mice – C. p. v. v. inoculation of 5Â105 C. albicans/ mouse. (For hints in choosing the inoculum size and strain of C. albicans, see Notes 2 and 3). 1. Recombinant Als1p-N/rAls3p-N Vaccine – IP Immunization; IV Challenge ( Refs.
2, is prewarmed to 50°C. 3. A moist dark incubation chamber (see Note 3). 1 The microbial species used in the artificial throat model Yeast species Bacterial species Candida albicans Streptococcus salivarius C. tropicalis Rothia dentocariosa Staphylococcus aureus S. 6. Confocal Laser Scanning Microscopy 49 1. CLSM model LEICA TCS SP2 (Leica Microsystems, Heidelberg GmbH, Heidelberg, Germany) 2. Lasers: UV laser, He-Ne laser, and Ar laser Wavelength: excitation 400 nm emission 490 (Calcofluor) excitation 488 nm emission 514 (FITC) excitation 543 emission 580 (Rhodamine) 3.
Candida albicans: Methods and Protocols by T. Sreevalsan (auth.), Ronald L. Cihlar, Richard A. Calderone (eds.)