By Barbara J. Bain
- allows either the haematologist and laboratory scientist to spot blood mobilephone beneficial properties, from the most typical to the extra obscure
- presents crucial info on equipment of assortment, blood movie coaching and marking, including the foundations of guide and automatic blood counts
- thoroughly revised and up to date, incorporating a lot newly released info: now comprises recommendation on additional assessments while a particular analysis is suspected
- four hundred prime quality images to assist with blood telephone identification
- Highlights the aim and medical relevance of haematology laboratory exams all through
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Additional resources for Blood Cells A Practical Guide, 5 edition
Sample text
A similar low cytometry technique has been recommended for routine platelet counts in severely thrombocytopenic patients, when an accurate platelet count is particularly important for determining whether or not a platelet transfusion is needed [29]. The differential white cell count A differential white cell count is the assigning of leucocytes to their individual categories, this categorisation being expressed as a percentage or, when the WBC is available, as an absolute count. The ICSH recommends that the differential leucocyte count be expressed in absolute numbers [30] and this is strongly recommended.
Alternatively, the platelet count can be determined by low cytometry using a luorochrome‐labelled monoclonal antibody, such as CD41, CD42a or CD61, that binds speciically to platelets. Either a known amount of luorescent beads can be used as a 23 calibrant, or the ratio of red cells to luorescent platelets can be determined and the platelet count can be calculated from a reference RBC [25–27]. The latter procedure is preferred, and is now the ICSH reference method [28], since dilution errors do not affect the count; a mixture of CD41 and CD61 directly-labelled antibodies is employed.
If NRBC are present, their percentage can be counted on a stained blood ilm and the total nucleated cell count (TNCC) can be corrected. The manual WBC is imprecise, but this is of less practical importance than the imprecision of the RBC, since clinically important changes in WBC are usually of suficient magnitude to be detected even with an imprecise method. White cells can also be counted in diluted whole blood following red cell lysis, using a single‐channel semi‐automated impedance counter.
Blood Cells A Practical Guide, 5 edition by Barbara J. Bain
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