By Herbert Begemann
-Enhanced sixth version comprises extra illustrations and documented figures
-A ideal reference for hematological and oncological departments and the clincal labortory
-Classified findings from the area well-being Organization
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Extra resources for Atlas of Clinical Hematology
Pipette the clearly visible leukocyte layer into a small pointed centrifuge tube, incubate this at 37° C for I h and centrifuge again carefully. Make several smears from the leukocyte layer and stain according to Pappenheim. In contrast to this direct method, in the indirect method serum or plasma of the patient is incubated with bone marrow blood or a concentrate of peripheral blood cells of a healthy person of the same blood group and then treated as above. Evaluation. A large number of leukocytes (best several 100-1,000) are counted and the observed LE cells noted.
Staining. 1 % aqueous erythrosin solution for 3 min. Examine under the microscope with a x 40 dry lens or in oil immersion. EMluation. HbA-containing erythrocytes are recognizable as weak shadows, the HbF-containing erythrocytes are bright red. The method is suitable for diagnosis of thalassemia major and the demonstration of fetal erythrocytes in the maternal circulation. 17 Demonstration of Methemoglohincontaining Erythrocytes in Blood Films (Kleihauer and Betke) Principle. Methemoglobin forms cyanmethemoglobin with KCN, whereas oxyhemoglobin does not react with cyanides.
Positive segmented nuclei (right) and negative monocyte. Method see p. 15 (b) Peroxidase reaction of peripheral blood cells by Graham-Knoll. Three positive segmented nuclei. Top right a negative lymphocyte (see also Table 5, p. 86). Method see p. II (c) Steinbrinck-Chediak-Higashi granulation anomaly (Preparation kindly supplied by Dr. P. Lopes Cardozo. Leiden) (d) Cytoplasm vacuoles in granulocytes 45 Fig. 11. Monocytes, macrophages -1,250 x The monocyte is an exceptionally polymorphous cell. -1m.
Atlas of Clinical Hematology by Herbert Begemann