By A H Rebar; et al
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Extra info for A Guide to Hematology in Dogs and Cats
Hemolytic disease. RBC with eccentric hemoglobin staining can be seen (eccentrocytes). Very little evidence of anisocytosis or polychromasia can be seen (60x). 38 TNM GuideToHema:03058 TNM text_cp 6/28/07 3:49 PM Page 39 4 E RY T H R O C Y T E S Figure 4-16 Canine blood. Hemolytic disease. Numerous eccentrocytes or “blister cells” are seen. These cells have an eccentric displacement of hemoglobin because opposing sides of the RBC membrane have fused due to oxidative injury. Causes of this change are identical to those that produce Heinz bodies (100x).
The degree of left shift cannot be determined from the QBC data. ® Tends to underestimate frequency of leukopenia. ® Algorithms used to calculate RBC and WBC counts assume normal cell size and structure. They are thus invalidated by conditions such as microcytosis, hypochromasia, and cell immaturity. Assessment of a stained blood smear is essential to rule out conditions that could lead to inaccurate differential counts. 25 TNM GuideToHema:03058 TNM text_cp 6/28/07 3:49 PM Page 26 Flow Cytometry (Figure 3-14) ® Laser flow cytometry is the newest and most accurate method of automated cell counting.
The poikilocytes in this blood smear have irregular membrane projections that have rounded tips. These cells are called acanthocytes (arrows) and represent an in vivo alteration rather than an artifact. Acanthocytes are frequently associated with hemangiosarcoma in the liver. A few spherocytes are also noted. (100x). ® Eccentrocytes – red cells with hemoglobin concentrated at one pole with an unstained area at the other pole (Figures 4-15 and 4-16). ® Schistocytes – irregularly shaped, often roughly triangular, red cell fragments (Figure 4-17).
A Guide to Hematology in Dogs and Cats by A H Rebar; et al